GeneTex
United States (US)

ERCC8 antibody [N2C2], Internal

GTX100145_WB_19011506_304.jpg
GTX100145_IFA_19011506_154.jpg
GTX100145_39384_WB_M_19011506_671.jpg

Cat No. GTX100145

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, Functional Assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Functional Assay Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , Neuro2A , GL261 , C8D30 , NIH-3T3, Raw264.7 , C2C12

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CKN1 antibody, CSA antibody, ERCC8 antibody, Cockayne syndrome WD-repeat protein CSA antibody, DNA excision repair protein ERCC-8 antibody, cockayne syndrome WD repeat protein CSA antibody, excision repair cross-complementing rodent repair deficiency, complementation group 8 antibody

Cellular Localization

Nucleus

Background

This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. [provided by RefSeq]

Database

DATA IMAGES
GTX100145_WB_19011506_304.jpg

GTX100145 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
12% SDS PAGE
GTX100145 diluted at 1:500

GTX100145_IFA_19011506_154.jpg

GTX100145 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8(CSA)(GTX100145) antibody at 1:200 dilution.

GTX100145_39384_WB_M_19011506_671.jpg

GTX100145 WB Image

ERCC8 antibody [N2C2], Internal detects ERCC8 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody [N2C2], Internal (GTX100145) dilution: 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169