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ERCC8 antibody

Anti-ERCC8 antibody used in Western Blot (WB). GTX100122
Anti-ERCC8 antibody used in Western Blot (WB). GTX100122
Anti-ERCC8 antibody used in Western Blot (WB). GTX100122
Anti-ERCC8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100122

Cat. No. GTX100122

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ERCC8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Molt-4 , C8D30 , Rat2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC8. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1950220

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERCC excision repair 8, CSA ubiquitin ligase complex subunit , CKN1 , CSA , UVSS2

Cellular Localization

Nucleus

Background

This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERCC8 antibody used in Western Blot (WB). GTX100122

GTX100122 WB Image

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
10% SDS PAGE
GTX100122 diluted at 1:1000

Anti-ERCC8 antibody used in Western Blot (WB). GTX100122

GTX100122 WB Image

ERCC8 antibody detects ERCC8 protein by Western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody (GTX100122) dilution: 1:1000

Anti-ERCC8 antibody used in Western Blot (WB). GTX100122

GTX100122 WB Image

ERCC8 antibody detects ERCC8 protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody (GTX100122) dilution: 1:1000

Anti-ERCC8 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100122

GTX100122 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ERCC8(GTX100122) antibody at 1:200 dilution.

REFERENCE

Application Reference

Huang Y et al. Cell Discov 2023; 9 (1) : 107 Heat shock protein DNAJA2 regulates transcription-coupled repair by triggering CSB degradation via chaperone-mediated autophagy.
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169