GeneTex
United States (US)

ERCC8 antibody

GTX100122_39932_WB_M_19011506_414.jpg
GTX100122_IFA_19011506_553.jpg
GTX100122_39932_WB_R_19011506_304.jpg
GTX100122_WB_19011506_378.jpg

Cat No. GTX100122

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Molt-4 , C8D30 , Rat2

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5.39mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERCC8. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CKN1 antibody, CSA antibody, ERCC8 antibody, Cockayne syndrome WD-repeat protein CSA antibody, DNA excision repair protein ERCC-8 antibody, cockayne syndrome WD repeat protein CSA antibody, excision repair cross-complementing rodent repair deficiency, complementation group 8 antibody

Cellular Localization

Nucleus

Background

This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. [provided by RefSeq]

Database

DATA IMAGES
GTX100122_39932_WB_M_19011506_414.jpg

GTX100122 WB Image

ERCC8 antibody detects ERCC8 protein by Western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody (GTX100122) dilution: 1:1000

GTX100122_IFA_19011506_553.jpg

GTX100122 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ERCC8(GTX100122) antibody at 1:200 dilution.

GTX100122_39932_WB_R_19011506_304.jpg

GTX100122 WB Image

ERCC8 antibody detects ERCC8 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody (GTX100122) dilution: 1:1000

GTX100122_WB_19011506_378.jpg

GTX100122 WB Image

Sample (30μg whole cell lysate)
A:MOLT4 (GTX27912)
10% SDS PAGE
GTX100122 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169