Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody (GTX114971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERF antibody detects ERF protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
ERF stained by ERF antibody (GTX114971) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ERF(GTX114971) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ERF antibody detects ERF protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERF stained by ERF antibody (GTX114971) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody (GTX114971) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.