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ERF antibody

Overexpression
Anti-ERF antibody used in Western Blot (WB). GTX114971
Anti-ERF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114971
Anti-ERF antibody used in IHC (Paraffin sections) (IHC-P). GTX114971
Anti-ERF antibody used in IHC (Paraffin sections) (IHC-P). GTX114971
Anti-ERF antibody used in Western Blot (WB). GTX114971

Cat. No. GTX114971

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ERF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

A431 , ERF-transfected 293T

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165552

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ETS2 repressor factor , CHYTS , CRS4 , PE-2 , PE2

Cellular Localization

Nucleus

Background

Members of the ETS family of transcription factors, such as ERF, regulate cell proliferation and differentiation. They share a highly conserved DNA-binding domain, the ETS domain, that recognizes the sequence GGAA/T (de Castro et al., 1997 [PubMed 9192842]). For further information on ETS transcription factors, see ETS1 (MIM 164720).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ERF antibody used in Western Blot (WB). GTX114971

GTX114971 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody (GTX114971) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114971

GTX114971 ICC/IF Image

ERF antibody detects ERF protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERF stained by ERF antibody (GTX114971) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

Anti-ERF antibody used in IHC (Paraffin sections) (IHC-P). GTX114971

GTX114971 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ERF(GTX114971) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERF antibody used in IHC (Paraffin sections) (IHC-P). GTX114971

GTX114971 IHC-P Image

ERF antibody detects ERF protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
ERF stained by ERF antibody (GTX114971) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ERF antibody used in Western Blot (WB). GTX114971

GTX114971 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERF antibody (GTX114971) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169