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ERG antibody

Anti-ERG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112925
Anti-ERG antibody used in Western Blot (WB). GTX112925
Anti-ERG antibody used in Western Blot (WB). GTX112925

Cat. No. GTX112925

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HUVEC,Raw264.7

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ERG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950222

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ETS transcription factor ERG , erg-3 , p55

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-ERG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112925

GTX112925 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ERG(GTX112925) antibody at 1:200 dilution.

Anti-ERG antibody used in Western Blot (WB). GTX112925

GTX112925 WB Image

ERG antibody detects ERG protein by Western blot analysis.
A. 30 μg Raw264.7 whole lysate/extract
10 % SDS-PAGE
ERG antibody (GTX112925) dilution: 1:1000

Anti-ERG antibody used in Western Blot (WB). GTX112925

GTX112925 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERG antibody (GTX112925) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169