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ERK1/2 antibody [E31R]

Anti-ERK1/2 antibody [E31R] used in Western Blot (WB). GTX82556

Cat. No. GTX82556

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 E31R

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all ERK1/2 products

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Product Note

Recognizes human ERK1 and ERK2 proteins

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human ERK1 protein

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_11173785

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , mitogenactivated protein kinase1 , ERK2 , mitogenactivated protein kinase3 , PRKM1 , mitogenactivated protein kinase 3 , p41MAPK , mitogenactivated protein kinase 1 , PRKM3 , ERK1 , mitogen-activated protein kinase 3 , P44MAPK , P44ERK1

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-ERK1/2 antibody [E31R] used in Western Blot (WB). GTX82556

GTX82556 WB Image

1:1000 (1μg/mL) Ab dilution used in WB of 10μg (1) and 30μg (2) HeLa cell lysates

REFERENCE

Application Reference

Li MY et al. Sci Rep 2018; 8 (1) : 14947 Ginkgolide B promotes neuronal differentiation through the Wnt/β-catenin pathway in neural stem cells of the postnatal mammalian subventricular zone.
Application : WB
Reactivity : Mouse
Hsu LH et al. Cancer Sci 2015; 106 (1) : 18872 Estrogen adversely affects the prognosis of patients with lung adenocarcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations