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ERK1/2 antibody

Comparison
Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462
Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462
Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462
Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462
Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462

Cat. No. GTX134462

Reference 19

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all ERK1/2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERK1/2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887288

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462

GTX134462 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/ERK2 antibody (GTX134462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462

GTX134462 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/ERK2 antibody (GTX134462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462

GTX134462 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERK1/2 antibody (GTX134462) diluted at 1:1000 and competitor's antibody (CST#9102) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462

GTX134462 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody (GTX134462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERK1/2 antibody used in Western Blot (WB). GTX134462

GTX134462 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK1/2 antibody (GTX134462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lee H et al. Nat Commun 2024; 15 (1) : 1003 R-Spondin 2 governs Xenopus left-right body axis formation by establishing an FGF signaling gradient.
Hung YH et al. Am J Cancer Res 2023; 13 (8) : 3417-3432 Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7) : e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Application : WB
Reactivity : Human
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Lee H et al. Nat Commun 2024; 15 (1):1003 R-Spondin 2 governs Xenopus left-right body axis formation by establishing an FGF signaling gradient.
Hung YH et al. Am J Cancer Res 2023; 13 (8):3417-3432 Pancreatic cancer cell-derived semaphorin 3A promotes neuron recruitment to accelerate tumor growth and dissemination.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Yan Sun et al. Immun Inflamm Dis 2022; 10 (7):e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Application : WB
Reactivity : Human
Elisa De Tomi et al. Int J Mol Sci 2022; 23 (3) Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.
Application : WB
Reactivity : Human
Lin NY et al. Oncogenesis 2022; 11 (1):8 C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma.
Application : WB
Su C.H. et al. Journal of Functional Foods 2021; 86 Betulin suppresses TNF-α and IL-1β production in osteoarthritis synovial fibroblasts by inhibiting the MEK/ERK/NF-κB pathway.
Application : WB
Reactivity : Human
Raineri A et al. Molecules. 2021; 26 (16):5027 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression.
Application : WB
Reactivity : Mouse
Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Tsai HJ et al. Sci Rep 2021; 11 (1):610 Angiotensin-(1-7) treatment blocks lipopolysaccharide-induced organ damage, platelet dysfunction, and IL-6 and nitric oxide production in rats.
Application : WB
Reactivity : Rat
Wang CH et al. Sci Rep 2020; 10 (1):2187 The novel application of cordycepin in maintaining stem cell pluripotency and increasing iPS cell generation efficiency.
Application : WB
Reactivity : Mouse
Wu YJ et al. Int J Med Sci 2020; 17 (3):403-413 Hinokitiol reduces tumor metastasis by inhibiting heparanase via extracellular signal-regulated kinase and protein kinase B pathway.
Application : WB
Reactivity : Mouse
Atzori MG et al. J Cell Mol Med 2020; 24 (1):465-475 Role of VEGFR-1 in melanoma acquired resistance to the BRAF inhibitor vemurafenib.
Application : WB
Reactivity : Human
Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
Lien HC et al. Pathology 2019; (Epub) Fibrillin-1, a novel TGF-beta-induced factor, is preferentially expressed in metaplastic carcinoma with spindle sarcomatous metaplasia.
Application : WB
Reactivity : Human
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : WB
Reactivity : Mouse
Huang KH et al. J Endod 2018; 44 (11):1677-1685 Enhanced Capability of Bone Morphogenetic Protein 2-loaded Mesoporous Calcium Silicate Scaffolds to Induce Odontogenic Differentiation of Human Dental Pulp Cells.
Application : WB
Reactivity : Human
Wang TH et al. Int J Mol Sci 2018; 19 (9) Melatonin Inhibits the Progression of Hepatocellular Carcinoma through MicroRNA Let7i-3p Mediated RAF1 Reduction.
Application : WB
Reactivity : Human
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169