WB analysis of 25 ug of various whole cell lysates using GTX15780 ERK1 antibody [12D11].
Dilution : 1:1000

ChIP analysis of 1x10⁶ HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes using GTX15780 ERK1 antibody [12D11]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 of Egr1.
IP reaction : 1.0ul antibody / 100ul sample

ICC/IF analysis of untreated and siRNA knockdown treated HeLa cells using GTX15780 ERK1 antibody [12D11].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:100 for at least 1 hour at room temperature

IHC-P analysis of human colon tissue using GTX15780 ERK1 antibody [12D11].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95ºC

ICC/IF analysis of A549 cells using GTX15780 ERK1 antibody [12D11]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature