1:1000 (1μg/mL) Ab dilution used in WB of HEK293 cell lysate; 5μg (1), 10μg (2), and 30μg (3) of cell lysate used

IP of anti-ERK1 (1μL) using HeLa cell lysate; 10μg (1), 25μg (2), and 50μg (3) of cell lysate used