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ERK1 antibody [E32]

Anti-ERK1 antibody [E32] used in (). GTX82558
Anti-ERK1 antibody [E32] used in Western Blot (WB). GTX82558

Cat. No. GTX82558

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 E32

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all ERK1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-10000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Calculated MW

43 kDa. ( Note )

Product Note

Recognizes only the ERK1 protein (e.g.: from human and mouse cells)

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length ERK1 protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 3 , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ERK1 antibody [E32] used in (). GTX82558

GTX82558 Image

IP of anti-ERK1 (1μL) using HeLa cell lysate; 10μg (1), 25μg (2), and 50μg (3) of cell lysate used

Anti-ERK1 antibody [E32] used in Western Blot (WB). GTX82558

GTX82558 WB Image

1:1000 (1μg/mL) Ab dilution used in WB of HEK293 cell lysate; 5μg (1), 10μg (2), and 30μg (3) of cell lysate used

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations