For IHC: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.
This antibody reacts with ERK1 p44 and to a lesser extent ERK2 p42 of mouse, rat, and human origin.
Phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide mapping within the subdomain XI of rat ERK1.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Mitogen Activated Protein Kinase 3,Erk1,Ert2,Erk-1,Esrk1,Mapk1,Mnk1,Prkm3,P44,P44Erk1,P44Mapk,Mapk3
MAP kinases consist of several subgroups, including the ERK, JNK, and p38 kinases. The members are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides (1). These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis (1,2). The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation (3). The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif (4,5). While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is also activated by dual phosphorylation at a T-G-Y motif (6,7).