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ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody

Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50274
Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in Western Blot (WB). GTX50274
Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in IHC (Paraffin sections) (IHC-P). GTX50274

Cat. No. GTX50274

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ERK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
IHC-Fr Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 202 (F-L-T(p)-E-Y) derived from human ERK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11172245

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , mitogen-activated protein kinase 3 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50274

GTX50274 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50274 ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody.

Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in Western Blot (WB). GTX50274

GTX50274 WB Image

WB analysis of extracts from 293 cells untreated or treated with PMA for the indicated times using GTX50274 ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody.

Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in IHC (Paraffin sections) (IHC-P). GTX50274

GTX50274 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50274 ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Yinghao Yu et al. J Clin Med 2021; 10 (15) The Paradoxical Effect Hypothesis of Abused Drugs in a Rat Model of Chronic Morphine Administration.
Application : IHC-Fr
Reactivity : Rat
Bai-Chuang Shyu et al. Front Aging Neurosci 2021; 13 : 751913 Methamphetamine and Modulation Functionality of the Prelimbic Cortex for Developing a Possible Treatment of Alzheimer's Disease in an Animal Model.
Application : IHC-Fr
Reactivity : Rat
Huang ACW et al. Neurobiol Learn Mem 2020; 172 : 107248 Interactions between prelimbic cortex and basolateral amygdala contribute to morphine-induced conditioned taste aversion in conditioning and extinction.
Application : WB
Reactivity : Mouse
He ABH et al. Neurobiol Learn Mem 2019; 166 : 107090 Involvement of neural substrates in reward and aversion to methamphetamine addiction: Testing the reward comparison hypothesis and the paradoxical effect hypothesis of abused drugs.
Application : IHC-Fr
Reactivity : Rat
Li HY et al. J Agric Food Chem 2019; 67 (7) : 1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399