GeneTex

  • Country / Location Selection

United States (US)

ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody

Orthogonal
Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in IHC (Paraffin sections) (IHC-P). GTX78988
Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in Western Blot (WB). GTX78988

Cat. No. GTX78988

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all ERK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of threonine 202 (F-L-Tp-E-Y).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11165650

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERK,ERK2,ERT1,MAPK1,MAPK2,P42MAPK,PRKM1,PRKM2,mitogenactivated protein kinase 1,p38,p40,p41,p41mapk,p42MAPK,P42 MAPK,mitogen-activated protein kinase 1

Cellular Localization

Cytoplasm,Cytoskeleton,Spindle,Nucleus,Microtubule organizing center,Centrosome,Membrane,Caveola

Background

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in IHC (Paraffin sections) (IHC-P). GTX78988

GTX78988 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78988 ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody.

Anti-ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody used in Western Blot (WB). GTX78988

GTX78988 WB Image

Orthogonal

WB analysis of NaCl or TNF alpha treated MCF7, 293, A431, A2780 and HeLa cell lysates using GTX78988 ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody (Lane 5, 6, 7, and 8) and ERK1/2 antibody (Lane 1, 2, 3, and 4).

REFERENCE

Application Reference

M Rokni et al. Iran J Allergy Asthma Immunol 2024; Interferon-γ Induces Interleukin-6 Production and Alpha-smooth Muscle Actin Expression in Systemic Sclerosis Fibroblasts
XinFu et al. BBA 2018; 1865 (11) : 1552-1565 Suppression of PTBP1 signaling is responsible for mesenchymal stem cell induced invasion of low malignancy cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for ERK1 (phospho Thr202) + ERK2 (phospho Thr185) antibody (GTX78988). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399