*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The antibody recognizes the same sequence in both proteins (ERK1/2) as long as the T and the Y are phosphorylated. Does not recognize either monophospho- forms of ERK1/2 or the non-phosphorylated proteins.
PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1 and 2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Caveola,Centrosome,Cytoplasm,Cytoskeleton,Membrane,Microtubule organizing center,Nucleus,Spindle
kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]