GeneTex
  • Country / Location Selection

United States (US)

ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

Cat No. GTX24819

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC, IHC-FrFl
Reactivity Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Xenopus
Package
50μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P 1:10-1:100
IHC Assay dependent
IHC-FrFl Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Specificity/Sensitivity

The antibody recognizes the same sequence in both proteins (ERK1/2) as long as the T and the Y are phosphorylated. Does not recognize either monophospho- forms of ERK1/2 or the non-phosphorylated proteins.
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ERK1 and 2 that contains threonine 202/185 and tyrosine 204/187. This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Caveola,Centrosome,Cytoplasm,Cytoskeleton,Membrane,Microtubule organizing center,Nucleus,Spindle

Background

kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]

Database

Research Area

DATA IMAGES
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of mouse stomach tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

WB analysis of NIH3T3 untreated (Lane 1) or treated with PDGF (Lane 2) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Loading: 5 ug

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

WB analysis of whole cell extracts (30 µg lysate) of MDA-MB-231 with treatment of EGF(100ng/ml for 15mins) (Lane 1), MDA-MB-231 (Lane 2), U-87 MG (Lane 3), SH-SY5Y (Lane 4) and HeLa (Lane 5) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Dilution : 1:1000

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX24819

GTX24819 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:50

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX24819

GTX24819 WB Image

WB (peptide competition) analysis of PC12 cells stimulated with 0.5 M sorbitol for 5 minutes (Lane 2-6) using GTX24819 ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphothreonine-containing peptide (Lane 4), a generic phosphotyrosine-containing peptide (Lane 5), or the phosphopeptide immunogen (Lane 6). The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE
Application Reference
Clone Reference
Package List Price ($)
$ 319