GeneTex

  • Country / Location Selection

United States (US)

ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX82696
Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX82696

Cat. No. GTX82696

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($499)
See all ERK1 products

APPLICATION

Application Note

Optimal working dilutions should be determined experimentally by the end user.

Calculated MW

42/44 kDa. ( Note )

Predict Reactivity

Mouse, Zebrafish, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

10mM HEPES, 150mM NaCl, 0.01% BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH.

Purification

Affinity Purified

Conjugation

Unconjugated

RRID

AB_11176163

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Mitogen-Activated Protein Kinase 3 , Erk-1 , Erk1 , Ert2 , Hs44Kdap , Humker1A , P44Erk1 , P44Mapk , Prkm3 , P44-Erk1 , P44-Mapk , Mapk3

Background

kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]

Research Area

DATA IMAGES

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in Western Blot (WB). GTX82696

GTX82696 WB Image

Western blot of human T47D cells showing phospho-specific immunolabeling of the ~42k-44k ERK/MAPK protein doublet using ERK/MAPK (phospho Thr202/Tyr204) antibody (GTX82696).

Anti-ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX82696

GTX82696 IHC-P Image

IHC-P analysis of granule cells in the dentate gyrus of saline treated mouse using ERK1/2 (phospho Thr202/Tyr204) antibody (red) and DAPI (blue).

REFERENCE

Application Reference

Yanxin Wei et al. Phytomedicine 2023; : 154980 Maintenance of airway epithelial barrier integrity via the inhibition of AHR/EGFR activation ameliorates chronic obstructive pulmonary disease using effective-component combination
Application : WB
Reactivity : Rat
Vazquez-Mellado MJ et al. Life Sci 2015; 137 (Epub) : 142-9 erythropoietin protects neuroblastoma cells against * and * by activating erk and akt pathways but has no effect in kidney cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for ERK1 (phospho Thr202/Tyr204) + ERK2 (phospho Thr185/Tyr187) antibody (GTX82696). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Package List Price ($)
$ 499