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ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody

Anti-ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50275
Anti-ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody used in Western Blot (WB). GTX50275
Anti-ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX50275

Cat. No. GTX50275

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all ERK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from human ERK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , mitogen-activated protein kinase 3 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50275

GTX50275 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50275 ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody.

Anti-ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody used in Western Blot (WB). GTX50275

GTX50275 WB Image

WB analysis of extracts from SK-BR-3 cells untreated, treated with insulin + EGF, U0126, or LY294002 using GTX50275 ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody.

Anti-ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody used in IHC (Paraffin sections) (IHC-P). GTX50275

GTX50275 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50275 ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399