ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody

Cat. No. GTX78987

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μg ($399)

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Datasheet

View product citations for antibody GTX78987 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human p44/42 MAP Kinase around the phosphorylation site of tyrosine 204 (T-E-Yp-V-A).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11169167

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERK,ERK2,ERT1,MAPK1,MAPK2,P42MAPK,PRKM1,PRKM2,mitogenactivated protein kinase 1,p38,p40,p41,p41mapk,p42MAPK,P42 MAPK,mitogen-activated protein kinase 1

Cellular Localization

Cytoplasm,Cytoskeleton,Spindle,Nucleus,Microtubule organizing center,Centrosome,Membrane,Caveola

Background

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]

Database

Gene ID: 5594 MAPK1
Gene ID: 5595
UniProt: P28482 MAPK1

Research Area

DATA IMAGES

GTX78987 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX78987 ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody.

GTX78987 WB Image

WB analysis of NIH-3T3 cell lysate using GTX78987 ERK1 (phospho Tyr204) + ERK2 (phospho Tyr187) antibody (Lane 3 and 4) and ERK1/2 antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

Qty