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ERK2 antibody [4C11]

Anti-ERK2 antibody [4C11] used in Western Blot (WB). GTX83148
Anti-ERK2 antibody [4C11] used in IHC (Paraffin sections) (IHC-P). GTX83148
Anti-ERK2 antibody [4C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83148

Cat. No. GTX83148

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4C11

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($419)
See all ERK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ERK2 expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ERK2 antibody [4C11] used in Western Blot (WB). GTX83148

GTX83148 WB Image

WB analysis of HeLa (1), NIH3T3 (2), MCF-7 (3), HEK293 (4), Jurkat (5), A549 (6), NTERA-2 (7) and SMMC-7721 (8) cell lysate using GTX83148 ERK2 antibody [4C11].

Anti-ERK2 antibody [4C11] used in IHC (Paraffin sections) (IHC-P). GTX83148

GTX83148 IHC-P Image

IHC-P analysis of human lung carcinoma (left) and breast carcinoma (right) using GTX83148 ERK2 antibody [4C11].

Anti-ERK2 antibody [4C11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83148

GTX83148 ICC/IF Image

ICC/IF analysis of Eca-109 cells using GTX83148 ERK2 antibody [4C11].
Green : ERK2
Red : Actin filament

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419