WB analysis of HEK293T cells transfected with ERK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84151 ERK2 antibody [4C2].
Loading : 5 ug per lane

IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84151 ERK2 antibody [4C2].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

WB analysis of various cell lines using GTX84151 ERK2 antibody [4C2].
Loading : 35 ug per lane

ICC/IF analysis of COS7 cells transiently transfected with ERK2 plasmid using GTX84151 ERK2 antibody [4C2].

WB analysis of wild type and ERK2 knock out HeLa cell lysate(10μg per lane) using GTX84151 ERK2 antibody [4C2].
Dilution : 1:500

IHC-P analysis of human pancreas tissue using GTX84151 ERK2 antibody [4C2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

ICC/IF analysis of COS7 cells using GTX84151 ERK2 antibody [4C2].