ERK2 antibody [6E5]

Name: ERK2 antibody [6E5]
Brand: GeneTex
SKU: GTX84159
Price: 379.00 USD

Anti-ERK2 antibody [6E5] used in Immunoprecipitation (IP). GTX84159

Anti-ERK2 antibody [6E5] used in IHC (Paraffin sections) (IHC-P). GTX84159

Anti-ERK2 antibody [6E5] used in Western Blot (WB). GTX84159

Cat No. GTX84159

Host	Mouse
Clonality	Monoclonal
Clone Name	6E5
Isotype	IgG1
Application	WB, IHC-P, IP
Reactivity	Human, Rat, Dog, Monkey
Package	100 μl ($379)

See all ERK2 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:2000
IHC-P	1:50
IP	2-4ug/mg

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

mitogen-activated protein kinase 1 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK

Cellular Localization

Nucleus,Cytoplasm

Background

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1 | required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Database

Gene ID: 5594 MAPK1
UniProt: P28482 MAPK1

Research Area

DATA IMAGES

GTX84159 IP Image

IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84159 ERK2 antibody [6E5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX84159 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84159 ERK2 antibody [6E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84159 IHC-P Image

IHC-P analysis of breast tissue using GTX84159 ERK2 antibody [6E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84159 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84159 ERK2 antibody [6E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84159 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84159 ERK2 antibody [6E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84159 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84159 ERK2 antibody [6E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84159 WB Image

WB analysis of HEK293T cells transfected with ERK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84159 ERK2 antibody [6E5].
Loading : 5 ug per lane

GTX84159 WB Image

WB analysis of wild type and ERK2 knock out HeLa cell lysate(10μg per lane) using GTX84159 ERK2 antibody [6E5].
Dilution : 1:500

GTX84159 WB Image

WB analysis of various cell lines using GTX84159 ERK2 antibody [6E5].
Loading : 35 ug per lane

GTX84159 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84159 ERK2 antibody [6E5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 379

Qty

ERK2 antibody [6E5]

Cat No. GTX84159

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84159 IP Image

GTX84159 IHC-P Image

GTX84159 IHC-P Image

GTX84159 IHC-P Image

GTX84159 IHC-P Image

GTX84159 IHC-P Image

GTX84159 WB Image

GTX84159 WB Image

GTX84159 WB Image

GTX84159 IHC-P Image

REFERENCE

REVIEW

Flyer - Neurotransmission

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