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ERK2 antibody [6F8]

KO/KD Overexpression
Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
Anti-ERK2 antibody [6F8] used in IHC (Paraffin sections) (IHC-P). GTX84154
Anti-ERK2 antibody [6F8] used in IHC (Paraffin sections) (IHC-P). GTX84154
Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
Anti-ERK2 antibody [6F8] used in Immunoprecipitation (IP). GTX84154
Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
Anti-ERK2 antibody [6F8] used in Flow cytometry (FACS). GTX84154

Cat. No. GTX84154

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6F8

Isotype

 IgG2a

Application

 WB, IHC-P, FACS, IP

Reactivity

 Human, Mouse, Rat, Dog, Monkey
Package
100 μl ($519)
See all ERK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human MAPK1(NP_620407) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK

Cellular Localization

Nucleus,Cytoplasm

Background

Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1 | required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Database

Research Area

DATA IMAGES

Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154

GTX84154 WB Image

KO/KD

WB analysis of wild type and ERK2 knock out HeLa cell lysate(10μg per lane) using GTX84154 ERK2 antibody [6F8].
Dilution : 1:500

Anti-ERK2 antibody [6F8] used in IHC (Paraffin sections) (IHC-P). GTX84154

GTX84154 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84154 ERK2 antibody [6F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-ERK2 antibody [6F8] used in IHC (Paraffin sections) (IHC-P). GTX84154

GTX84154 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84154 ERK2 antibody [6F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154

GTX84154 WB Image

WB analysis of various cell lines using GTX84154 ERK2 antibody [6F8].
Loading : 35 ug per lane

Anti-ERK2 antibody [6F8] used in Immunoprecipitation (IP). GTX84154

GTX84154 IP Image

IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84154 ERK2 antibody [6F8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154

GTX84154 WB Image

Overexpression

WB analysis of HEK293T cells transfected with ERK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84154 ERK2 antibody [6F8].
Loading : 5 ug per lane

Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154

GTX84154 WB Image

WB analysis of various samples using GTX84154 ERK2 antibody [6F8].
Loading : 10 ug per lane
Dilution : 1:200

Anti-ERK2 antibody [6F8] used in Flow cytometry (FACS). GTX84154

GTX84154 FACS Image

FACS analysis of HEK293T cells transfected with either ERK2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84154 ERK2 antibody [6F8].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519