ERK2 antibody [6F8]

Cat. No. GTX84154
Cat. No.  GTX84154
KO/KD Overexpression
Anti-ERK2 antibody [6F8] used in Flow cytometry (FCM). GTX84154
GTX84154 FCM Image

FACS analysis of HEK293T cells transfected with either ERK2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84154 ERK2 antibody [6F8].

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Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
GTX84154 WB Image

WB analysis of various samples using GTX84154 ERK2 antibody [6F8].
Loading : 10 ug per lane
Dilution : 1:200

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Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
GTX84154 WB Image
Overexpression

WB analysis of HEK293T cells transfected with ERK2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84154 ERK2 antibody [6F8].
Loading : 5 ug per lane

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Anti-ERK2 antibody [6F8] used in Immunoprecipitation (IP). GTX84154
GTX84154 IP Image

IP analysis of DDDDK tagged ERK2 overexpressed HEK293T lysate using GTX84154 ERK2 antibody [6F8].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

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Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
GTX84154 WB Image

WB analysis of various cell lines using GTX84154 ERK2 antibody [6F8].
Loading : 35 ug per lane

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Anti-ERK2 antibody [6F8] used in IHC (Paraffin sections) (IHC-P). GTX84154
GTX84154 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84154 ERK2 antibody [6F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

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Anti-ERK2 antibody [6F8] used in IHC (Paraffin sections) (IHC-P). GTX84154
GTX84154 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84154 ERK2 antibody [6F8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

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Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
GTX84154 WB Image
KO/KD

WB analysis of wild type and ERK2 knock out HeLa cell lysate(10μg per lane) using GTX84154 ERK2 antibody [6F8].
Dilution : 1:500

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Anti-ERK2 antibody [6F8] used in Flow cytometry (FCM). GTX84154
Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
Anti-ERK2 antibody [6F8] used in Immunoprecipitation (IP). GTX84154
Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
Anti-ERK2 antibody [6F8] used in IHC (Paraffin sections) (IHC-P). GTX84154
Anti-ERK2 antibody [6F8] used in IHC (Paraffin sections) (IHC-P). GTX84154
Anti-ERK2 antibody [6F8] used in Western Blot (WB). GTX84154
  • Host
    Mouse
  • Clonality
    Monoclonal
  • Clone Name
    6F8
  • Isotype
    IgG2a
  • Applications
    WB IHC-P FCM IP
  • Reactivity
    Human, Mouse, Rat, Dog, Monkey
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