Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [GT1183] (GTX00945) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of various samples using GTX00945 ERK2 antibody [GT1183].
Dilution : 1:1000
Loading : 25μg

WB analysis of wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts using GTX00945 ERK2 antibody [GT1183].
Dilution : 1:1000
Laoding : 25μg