ERK2 antibody [N1], N-term

Name: ERK2 antibody [N1], N-term
Brand: GeneTex
SKU: GTX104613
Price: 419.00 USD
Rating: 5 (1 reviews)

Anti-ERK2 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104613

Anti-ERK2 antibody [N1], N-term used in Western Blot (WB). GTX104613

Anti-ERK2 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104613

Cat. No. GTX104613

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Cat, Dog
Package	100 μl ($419), 25 μl ($169)

See all ERK2 products

Datasheet
Document

View product citations for antibody GTX104613 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

A431 , H1299 , HepG2 , HeLa

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ERK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240776

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 1 , ERK , ERK-2 , ERK2 , ERT1 , MAPK2 , P42MAPK , PRKM1 , PRKM2 , p38 , p40 , p41 , p41mapk , p42-MAPK

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]

Database

Gene ID: 5594 MAPK1
UniProt: P28482 MAPK1

Research Area

DATA IMAGES

GTX104613 IHC-P Image

ERK2 antibody [N1], N-term detects ERK2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
ERK2 antibody [N1], N-term (GTX104613) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104613 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: HepG2
12% SDS PAGE
GTX104613 diluted at 1:1500

GTX104613 WB Image

Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N1], N-term (GTX104613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104613 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody (GTX104613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX104613 WB Image

GTX104613 IHC-P Image

ERK2 antibody detects ERK2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
ERK2 stained by ERK2 antibody (GTX104613) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104613 ICC/IF Image

ERK2 antibody detects ERK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERK2 stained by ERK2 antibody (GTX104613) diluted at 1:500.
Blue: Hoechst 33342 staining.

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