GeneTex
United States (US)

ERK2 antibody [N1], N-term

KO/KD
GTX104613_39624_20160518_IHC-P_H_19011506_686.jpg
GTX104613_39624_WB_19011506_923.jpg
GTX104613_39624_20170330_WB_KO_watermark_19011506_518.jpg

Cat No. GTX104613

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HeLa , 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , *Mal19

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ERK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase 1,Erk,Erk-2,Erk2,Ert1,Mapk2,P42Mapk,Prkm1,Prkm2,P38,P40,P41,P41Mapk,P42-Mapk,Mapk1

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104613_39624_20160518_IHC-P_H_19011506_686.jpg

GTX104613 IHC-P Image

ERK2 antibody [N1], N-term detects ERK2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
ERK2 antibody [N1], N-term (GTX104613) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104613_39624_WB_19011506_923.jpg

GTX104613 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: HepG2
12% SDS PAGE
GTX104613 diluted at 1:1500

GTX104613_39624_20170330_WB_KO_watermark_19011506_518.jpg

GTX104613 WB Image

KO/KD

Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N1], N-term (GTX104613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

REVIEW

ERK2 antibody [N1], N-term Cat No. GTX104613

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 05-Mar-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Mal19 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169