Wild-type (WT) and ERK2 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody [N1], N-term (GTX104613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: HepG2
12% SDS PAGE
GTX104613 diluted at 1:1500

ERK2 antibody [N1], N-term detects ERK2 protein at cytoplasm and nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
ERK2 antibody [N1], N-term (GTX104613) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ERK2 antibody detects ERK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERK2 stained by ERK2 antibody (GTX104613) diluted at 1:500.
Blue: Hoechst 33342 staining.

ERK2 antibody detects ERK2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
ERK2 stained by ERK2 antibody (GTX104613) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody (GTX104613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERK2 antibody (GTX104613) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.