GeneTex
United States (US)

ERK2 antibody

GTX27948_18121410_196.jpg

Cat No. GTX27948

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For IHC: Use at an assay dependent dilution. For IP: Use at an assay dependent dilution. For WB: Use at a dilution of 1:400. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

41 kDa. ( Note )

Positive Control

A431, HeLa, HL-60, and NIH/3T3

Specificity/Sensitivity

This antibody reacts with ERK 2 p44 and to a lesser extent ERK 1 p42 of human, mouse, and rat origin.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.1% sodium azide and 0.2% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide (Rat) (C terminal).

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

MAP kinases consist of several subgroups, including the ERK, JNK, and p38 kinases. The members are regulated by many different extracellular cues ranging from cytokines, growth factors, and neuropeptides (1). These stimuli activate cell surface receptors to stresses such as cold, heat, osmolarity changes and irradiation. The pathways regulated by the MAPKs control a broad array of cellular responses ranging from survival, cell proliferation, and apoptosis (1,2). The MAPKs family is also characterized by their requirement for dual phosphorylation at a conserved threonine and tyrosine residue for enzymatic activation and both must be phosphorylated for full enzymatic activation (3). The closely related ERK1 (44 kDa) and ERK2 (42 kDa) kinases are characterized by their requirement for dual phosphorylation at a conserved T-E-Y motif (4,5). While JNK1 is activated by dual phosphorylation at a T-P-Y motif and p38 is also activated by dual phosphorylation at a T-G-Y motif (6,7).

Database

Research Area

DATA IMAGES
GTX27948_18121410_196.jpg

GTX27948 WB Image


REFERENCE