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ERK3 antibody [5E1]

Overexpression
Anti-ERK3 antibody [5E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84146
Anti-ERK3 antibody [5E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84146
Anti-ERK3 antibody [5E1] used in Western Blot (WB). GTX84146
Anti-ERK3 antibody [5E1] used in Western Blot (WB). GTX84146

Cat. No. GTX84146

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5E1

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($509)
See all ERK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 6 , ERK3 , HsT17250 , PRKM6 , p97MAPK

Cellular Localization

Cytoplasm,Nucleus

Background

Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.

Database

Research Area

DATA IMAGES

Anti-ERK3 antibody [5E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84146

GTX84146 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with ERK3 plasmid using GTX84146 ERK3 antibody [5E1].

Anti-ERK3 antibody [5E1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84146

GTX84146 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84146 ERK3 antibody [5E1].

Anti-ERK3 antibody [5E1] used in Western Blot (WB). GTX84146

GTX84146 WB Image

WB analysis of A549 cell lysate using GTX84146 ERK3 antibody [5E1].
Loading : 10 ug per lane
Dilution : 1:200

Anti-ERK3 antibody [5E1] used in Western Blot (WB). GTX84146

GTX84146 WB Image

Overexpression

WB analysis of HEK293T cells transfected with ERK3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84146 ERK3 antibody [5E1].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 509