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ERK3 antibody

KO/KD
Anti-ERK3 antibody used in Western Blot (WB). GTX35227
Anti-ERK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35227
Anti-ERK3 antibody used in Western Blot (WB). GTX35227

Cat. No. GTX35227

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all ERK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 372-721 of human MAPK6 (NP_002739.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 6 , ERK3 , HsT17250 , PRKM6 , p97MAPK

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ERK3 antibody used in Western Blot (WB). GTX35227

GTX35227 WB Image

WB analysis of various sample lysates using GTX35227 ERK3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ERK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35227

GTX35227 ICC/IF Image

ICC/IF analysis of L929 cells using GTX35227 ERK3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ERK3 antibody used in Western Blot (WB). GTX35227

GTX35227 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35227 ERK3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399