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ERK5 antibody

Anti-ERK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55608
Anti-ERK5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55608
Anti-ERK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55608
Anti-ERK5 antibody used in Western Blot (WB). GTX55608

Cat. No. GTX55608

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ERK5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human MAPK7 (NP_002740.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase 7 , BMK1 , ERK4 , ERK5 , PRKM7

Cellular Localization

Cytoplasm,Nucleus

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-ERK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55608

GTX55608 IHC-P Image

IHC-P analysis of rat liver tissue using GTX55608 ERK5 antibody.
Dilution : 1:50

Anti-ERK5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55608

GTX55608 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX55608 ERK5 antibody.
Blue : DAPI

Anti-ERK5 antibody used in IHC (Paraffin sections) (IHC-P). GTX55608

GTX55608 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX55608 ERK5 antibody.
Dilution : 1:50

Anti-ERK5 antibody used in Western Blot (WB). GTX55608

GTX55608 WB Image

WB analysis of various sample lysates using GTX55608 ERK5 antibody.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399