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ERM antibody

Anti-ERM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114394
Anti-ERM antibody used in IHC (Paraffin sections) (IHC-P). GTX114394
Anti-ERM antibody used in IHC (Paraffin sections) (IHC-P). GTX114394
Anti-ERM antibody used in Western Blot (WB). GTX114394
Anti-ERM antibody used in Western Blot (WB). GTX114394

Cat No. GTX114394

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Observed MW

75 kDa. The observed M.W. is based on the following publication. PMID: 20826535

Positive Control

ERM-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619907

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ETS variant 5 , ERM

Cellular Localization

Nucleus

Background

Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.

Database

Research Area

DATA IMAGES

Anti-ERM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114394

GTX114394 ICC/IF Image

ERM antibody detects ERM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERM protein stained by ERM antibody (GTX114394) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-ERM antibody used in IHC (Paraffin sections) (IHC-P). GTX114394

GTX114394 IHC-P Image

ERM antibody detects ERM protein at nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ERM antibody (GTX114394) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ERM antibody used in IHC (Paraffin sections) (IHC-P). GTX114394

GTX114394 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ERM(GTX114394) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERM antibody used in Western Blot (WB). GTX114394

GTX114394 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERM antibody used in Western Blot (WB). GTX114394

GTX114394 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

ERM antibody Cat No. GTX114394

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 02-Sep-2019
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : dorsal root ganglion
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.3% Triton X-100 in PBS, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 6Hr
Figure Note : Mouse dorsal root ganglion. ERM (left) and nuclei (right).
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf