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ERM antibody

Orthogonal Overexpression
Anti-ERM antibody used in Western Blot (WB). GTX114394
Anti-ERM antibody used in Western Blot (WB). GTX114394
Anti-ERM antibody used in Western Blot (WB). GTX114394
Anti-ERM antibody used in Western Blot (WB). GTX114394
Anti-ERM antibody used in IHC (Paraffin sections) (IHC-P). GTX114394
Anti-ERM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114394

Cat. No. GTX114394

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ERM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Observed MW

75 kDa. The observed M.W. is based on the following publication. PMID: 20826535

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619907

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ETS variant 5 , ERM

Cellular Localization

Nucleus

Background

Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.

Database

Research Area

DATA IMAGES

Anti-ERM antibody used in Western Blot (WB). GTX114394

GTX114394 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ERM antibody used in Western Blot (WB). GTX114394

GTX114394 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERM antibody used in Western Blot (WB). GTX114394

GTX114394 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERM antibody used in Western Blot (WB). GTX114394

GTX114394 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERM antibody used in IHC (Paraffin sections) (IHC-P). GTX114394

GTX114394 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ERM(GTX114394) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114394

GTX114394 ICC/IF Image

ERM antibody detects ERM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERM protein stained by ERM antibody (GTX114394) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Yamaji K et al. Dev Dyn 2018; 247 (11) : 1175-1185 Maldevelopment of the submandibular gland in a mouse model of apert syndrome.
Application : IHC-P
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169