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ERP29 antibody

Anti-ERP29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102225
Anti-ERP29 antibody used in Western Blot (WB). GTX102225
Anti-ERP29 antibody used in Western Blot (WB). GTX102225
Anti-ERP29 antibody used in IHC (Paraffin sections) (IHC-P). GTX102225
Anti-ERP29 antibody used in IHC (Paraffin sections) (IHC-P). GTX102225
Anti-ERP29 antibody used in IHC (Paraffin sections) (IHC-P). GTX102225
Anti-ERP29 antibody used in IHC (Paraffin sections) (IHC-P). GTX102225

Cat. No. GTX102225

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ERP29 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , H1299

Predict Reactivity

Sheep, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERP29. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950227

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

endoplasmic reticulum protein 29 , C12orf8 , ERp28 , ERp31 , HEL-S-107 , PDI-DB , PDIA9

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERP29 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102225

GTX102225 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ERP29(GTX102225) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-ERP29 antibody used in Western Blot (WB). GTX102225

GTX102225 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX102225 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERP29 antibody used in Western Blot (WB). GTX102225

GTX102225 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
12% SDS PAGE
GTX102225 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERP29 antibody used in IHC (Paraffin sections) (IHC-P). GTX102225

GTX102225 IHC-P Image

ERP29 antibody detects ERP29 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
ERP29 antibody (GTX102225) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERP29 antibody used in IHC (Paraffin sections) (IHC-P). GTX102225

GTX102225 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using ERP29(GTX102225) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERP29 antibody used in IHC (Paraffin sections) (IHC-P). GTX102225

GTX102225 IHC-P Image

ERP29 antibody detects ERP29 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ERP29 antibody (GTX102225) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERP29 antibody used in IHC (Paraffin sections) (IHC-P). GTX102225

GTX102225 IHC-P Image

ERP29 antibody detects ERP29 protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ERP29 antibody (GTX102225) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Guo L et al. Oncol Rep 2019; 41 (3) : 1603-1615 ERp29 counteracts the suppression of malignancy mediated by endoplasmic reticulum stress and promotes the metastasis of colorectal cancer.
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169