GeneTex
United States (US)

ERP72 antibody

KO/KD Overexpression
GTX115263_40527_20181109_WB_KO_watermark_19011519_913.jpg
GTX115263_40527_IHC_18121410_978.jpg
GTX115263_40527_WB_M_18121410_556.jpg
GTX115263_40527_WB_18121410_869.jpg
GTX115263_40527_IFA_18121410_825.jpg

Cat No. GTX115263

Reference 3
1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
See all ERP72 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , A431 , NIH-3T3 , JC , BCL-1

Specificity/Sensitivity

Knockdown/Knockout & overexpression validation were supported by customer review data.

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ERP72. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Disulfide Isomerase Family A Member 4,Erp70,Erp72,Erp-72,Pdia4

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Database

DATA IMAGES
GTX115263_40527_20181109_WB_KO_watermark_19011519_913.jpg

GTX115263 WB Image

KO/KD

Wild-type (WT) and ERP72 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERP72 antibody (GTX115263) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX115263_40527_IHC_18121410_978.jpg

GTX115263 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ERP72(GTX115263) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX115263_40527_WB_M_18121410_556.jpg

GTX115263 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX115263 diluted at 1:10000

GTX115263_40527_WB_18121410_869.jpg

GTX115263 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX115263 diluted at 1:1000

GTX115263_40527_IFA_18121410_825.jpg

GTX115263 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ERP72(GTX115263) antibody (Green) at 1:500 dilution.
REFERENCE

Application Reference

Sekiguchi T et al. Genes Cells 2019; 24 (2) : 151-161 RagA, an mTORC1 activator, interacts with a hedgehog signaling protein, WDR35/IFT121.
Application : WB
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Kadokura H et al. Biochem Biophys Res Commun 2013; 440 (2) : 245-50 Identification of the redox partners of ERdj5/JPDI, a PDI family member, from an animal tissue.
Application : IP
Reactivity : Mouse
Submit a Reference
REVIEW

ERP72 antibody Cat No. GTX115263

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 02-Nov-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T, HeLa cells
Amount used : 10 μg
Blocking : Blocking One, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169