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ERP72 antibody

KO/KD Comparison Overexpression
Anti-ERP72 antibody used in Western Blot (WB). GTX115263
Anti-ERP72 antibody used in Western Blot (WB). GTX115263
Anti-ERP72 antibody used in IHC (Paraffin sections) (IHC-P). GTX115263
Anti-ERP72 antibody used in Western Blot (WB). GTX115263
Anti-ERP72 antibody used in Western Blot (WB). GTX115263
Anti-ERP72 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115263

Cat. No. GTX115263

Reference 5
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ERP72 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

293T , A431 , NIH-3T3 , JC , BCL-1 , 293T

Product Note

Knockdown/Knockout & overexpression validation were supported by customer review data.

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ERP72. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11161939

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein disulfide isomerase family A member 4 , ERP70 , ERP72 , ERp-72

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Database

Research Area

DATA IMAGES

Anti-ERP72 antibody used in Western Blot (WB). GTX115263

GTX115263 WB Image

KO/KD

Wild-type (WT) and ERP72 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERP72 antibody (GTX115263) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERP72 antibody used in Western Blot (WB). GTX115263

GTX115263 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with ERP72 antibody (GTX115263) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ERP72 antibody used in IHC (Paraffin sections) (IHC-P). GTX115263

GTX115263 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using ERP72(GTX115263) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERP72 antibody used in Western Blot (WB). GTX115263

GTX115263 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX115263 diluted at 1:10000

Anti-ERP72 antibody used in Western Blot (WB). GTX115263

GTX115263 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
7.5% SDS PAGE
GTX115263 diluted at 1:1000

Anti-ERP72 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115263

GTX115263 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ERP72(GTX115263) antibody (Green) at 1:500 dilution.

REFERENCE

Application Reference

Kanemura S et al. J Biol Chem 2020; 295 (36) : 12772-12785 Characterization of the endoplasmic reticulum-resident peroxidases GPx7 and GPx8 shows the higher oxidative activity of GPx7 and its linkage to oxidative protein folding.
Application : WB
Shingo Kanemura et al. bioRxiv 2020; (Epub) Biochemical characterizations of ER-resident peroxidases, GPx7 and GPx8, reveal their distinct and regulated oxidative activities.
Application : WB
Sekiguchi T et al. Genes Cells 2019; 24 (2) : 151-161 RagA, an mTORC1 activator, interacts with a hedgehog signaling protein, WDR35/IFT121.
Application : WB
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Kadokura H et al. Biochem Biophys Res Commun 2013; 440 (2) : 245-50 Identification of the redox partners of ERdj5/JPDI, a PDI family member, from an animal tissue.
Application : IP
Reactivity : Mouse
Submit a Reference

REVIEW

ERP72 antibody Cat. No. GTX115263

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ERP72 antibody used in Western Blot (WB). GTX115263
Date : Anonymous submitted on 02-Nov-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T, HeLa cells
Amount used : 10 μg
Blocking : Blocking One, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169