*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant protein encompassing a sequence within the center region of human ERR alpha. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ERR1 antibody, ERRa antibody, ERRalpha antibody, ESRL1 antibody, NR3B1 antibody, ESRRA antibody, estrogen-related nuclear receptor alpha antibody, estrogen receptor-like 1 antibody, steroid hormone receptor ERR1 antibody, nuclear receptor subfamily 3 group B member 1 antibody, ERR-alpha antibody, estrogen-related receptor alpha antibody
The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq]
GTX106233 ChIP assay Image
Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.
GTX106233 WB Image
Sample (30 ug of whole cell lysate)
10% SDS PAGE
GTX106233 diluted at 1:2000
GTX106233 IP Image
ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 μg of ERR alpha antibody (GTX106233) 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (GTX106233) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.