GeneTex
United States (US)

ERR alpha antibody [N1C3]

GTX106233_40471_ChIP_19011506_874.jpg
GTX106233_40471_WB_19011506_308.jpg
GTX106233_40471_IP_19011506_758.jpg

Cat No. GTX106233

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ERR alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ERR1 antibody, ERRa antibody, ERRalpha antibody, ESRL1 antibody, NR3B1 antibody, ESRRA antibody, estrogen-related nuclear receptor alpha antibody, estrogen receptor-like 1 antibody, steroid hormone receptor ERR1 antibody, nuclear receptor subfamily 3 group B member 1 antibody, ERR-alpha antibody, estrogen-related receptor alpha antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq]
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Database

DATA IMAGES
GTX106233_40471_ChIP_19011506_874.jpg

GTX106233 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.

GTX106233_40471_WB_19011506_308.jpg

GTX106233 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX106233 diluted at 1:2000

GTX106233_40471_IP_19011506_758.jpg

GTX106233 IP Image

ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 μg of ERR alpha antibody (GTX106233) 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (GTX106233) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169