APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT9E9 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDIA5 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein disulfide isomerase family A member 3 , ER60 , ERp57 , ERp60 , ERp61 , GRP57 , GRP58 , HELS269 , HELS93n , HsT17083 , P58 , PDIA3 , PIPLC
Cellular Localization
Endoplasmic reticulum,Endoplasmic reticulum lumen,Melanosome
Background
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
![Anti-ERp57 antibody [AT9E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57663](/templates/default/js/lightbox/images/loading.gif "Anti-ERp57 antibody [AT9E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57663")
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GTX57663 ICC/IF Image
ICC/IF analysis of Hep3B cells using GTX57663 ERp57 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57663 WB Image
WB analysis of various samples using GTX57663 ERp57 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : NIH-3T3 whole cell lysate
Lane 4 : Raw 264.7 whole cell lysate
Lane 5 : LNCap whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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REFERENCE
There are currently no references for ERp57 antibody [AT9E9] (GTX57663). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ERp57 antibody [AT9E9] (GTX57663). Be the first to share your experience with this product.
![Anti-ERp57 antibody [AT9E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57663](/upload/website/prouct_img/small/GTX57663/GTX57663_20200204_IF_w_23061123_550.webp "Anti-ERp57 antibody [AT9E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57663")
![Anti-ERp57 antibody [AT9E9] used in Western Blot (WB). GTX57663](/upload/website/prouct_img/small/GTX57663/GTX57663_20200204_WB_w_23061123_699.webp "Anti-ERp57 antibody [AT9E9] used in Western Blot (WB). GTX57663")
![Anti-ERp57 antibody [AT9E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57663](/upload/website/prouct_img/normal/GTX57663/GTX57663_20200204_IF_w_23061123_550.webp "Anti-ERp57 antibody [AT9E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57663")
![Anti-ERp57 antibody [AT9E9] used in Western Blot (WB). GTX57663](/upload/website/prouct_img/normal/GTX57663/GTX57663_20200204_WB_w_23061123_699.webp "Anti-ERp57 antibody [AT9E9] used in Western Blot (WB). GTX57663")