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ERp57 antibody [C3], C-term

Comparison
Anti-ERp57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100297
Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297
Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297
Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297
Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297
Anti-ERp57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100297
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

Cat. No. GTX100297

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ERp57 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , H1299 , HeLa , HepG2

Predict Reactivity

Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERp57. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1081002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein disulfide isomerase family A member 3 , ER60 , ERp57 , ERp60 , ERp61 , GRP57 , GRP58 , HEL-S-269 , HEL-S-93n , HsT17083 , P58 , PI-PLC

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ERp57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100297

GTX100297 ICC/IF Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERp57 protein stained by ERp57 antibody [C3], C-term (GTX100297) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297

GTX100297 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100297 diluted at 1:10000

Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297

GTX100297 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERp57 antibody [C3], C-term (GTX100297) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297

GTX100297 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term (GTX100297) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297

GTX100297 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX100297 diluted at 1:1000

Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

GTX100297 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using ERp57(GTX100297) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERp57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100297

GTX100297 ICC/IF Image

ERp57 antibody [C3], C-term detects PDIA3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: PDIA3 protein stained by ERp57 antibody [C3], C-term (GTX100297) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kanemura S et al. J Biol Chem 2020; 295 (36) : 12772-12785 Characterization of the endoplasmic reticulum-resident peroxidases GPx7 and GPx8 shows the higher oxidative activity of GPx7 and its linkage to oxidative protein folding.
Application : WB
Shingo Kanemura et al. bioRxiv 2020; (Epub) Biochemical characterizations of ER-resident peroxidases, GPx7 and GPx8, reveal their distinct and regulated oxidative activities.
Application : WB
Teodorof C et al. Neurobiol Dis 2014; Intracellular mannose binding lectin mediates subcellular trafficking of HIV-1 gp120 in neurons.
Application : ICC/IF
Reactivity : Human
Chen YS et al. Biochem Biophys Res Commun 2013; 440 (4) : 671-6 Identification of the proteins required for fatty acid desaturation in zebrafish (Danio rerio).
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169