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ERp57 antibody [C3], C-term

Anti-ERp57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100297
Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297
Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297
Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297
Anti-ERp57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100297
Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297

Cat No. GTX100297

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

H1299 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , rat liver

Predict Reactivity

Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERp57. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

protein disulfide isomerase family A member 3,ER60,ERp57,ERp60,ERp61,GRP57,GRP58,HEL-S-269,HEL-S-93n,HsT17083,P58,PI-PLC

Cellular Localization

Endoplasmic reticulum lumen , Melanosome

Background

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ERp57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100297

GTX100297 ICC/IF Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERp57 protein stained by ERp57 antibody [C3], C-term (GTX100297) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297

GTX100297 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100297 diluted at 1:10000

Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

GTX100297 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using ERp57(GTX100297) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERp57 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100297

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297

GTX100297 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX100297 diluted at 1:1000

Anti-ERp57 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100297

GTX100297 ICC/IF Image

ERp57 antibody [C3], C-term detects PDIA3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: PDIA3 protein stained by ERp57 antibody [C3], C-term (GTX100297) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-ERp57 antibody [C3], C-term used in Western Blot (WB). GTX100297

GTX100297 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody [C3], C-term (GTX100297) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169