GeneTex
United States (US)

ERp57 antibody [C3], C-term

GTX100297_39422_20150410_IFA_19011506_451.jpg
GTX100297_WB_19011506_762.jpg
GTX100297_39422_IHC_M_19011506_599.jpg
GTX100297_39422_IHC_M_2_19011506_402.jpg
GTX100297_39428_WB_R_liver_19011506_555.jpg
GTX100297_IHC_19011506_789.jpg
GTX100297_39422_IHC_R_19011506_963.jpg
GTX100297_39422_WB_M_19011506_473.jpg
GTX100297_39428_IFA_19011506_609.jpg

Cat No. GTX100297

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

H1299 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , rat liver

Predict Reactivity

Sheep, Bovine, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERp57. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ER60 antibody, ERp57 antibody, ERp60 antibody, ERp61 antibody, GRP57 antibody, GRP58 antibody, HsT17083 antibody, P58 antibody, PI-PLC antibody, PDIA3 antibody, protein disulfide isomerase-associated 3 antibody, ER protein 60 antibody, 58 kDa glucose-regulated protein antibody, endoplasmic reticulum resident protein 60 antibody, phospholipase C-alpha antibody, ER protein 57 antibody, endoplasmic reticulum P58 antibody, glucose regulated protein, 58kDa antibody, disulfide isomerase ER-60 antibody, endoplasmic reticulum resident protein 57 antibody, protein disulfide-isomerase A3 antibody, 58 kDa microsomal protein antibody, protein disulfide isomerase family A, member 3 antibody

Cellular Localization

Endoplasmic reticulum lumen (By similarity) , Melanosome

Background

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100297_39422_20150410_IFA_19011506_451.jpg

GTX100297 ICC/IF Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERp57 protein stained by ERp57 antibody [C3], C-term (GTX100297) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX100297_WB_19011506_762.jpg

GTX100297 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Hela
C: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100297 diluted at 1:10000

GTX100297_39422_IHC_M_19011506_599.jpg

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on mouse lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100297_39422_IHC_M_2_19011506_402.jpg

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100297_39428_WB_R_liver_19011506_555.jpg

GTX100297 WB Image

ERp57 antibody [C3], C-term detects PDIA3 protein by Western blot analysis.
A. 20 µg rat liver lysate/extract
7.5 % SDS-PAGE
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:10000

GTX100297_IHC_19011506_789.jpg

GTX100297 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using ERp57(GTX100297) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100297_39422_IHC_R_19011506_963.jpg

GTX100297 IHC-P Image

ERp57 antibody [C3], C-term detects ERp57 protein at cytosol on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
ERp57 antibody [C3], C-term (GTX100297) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100297_39422_WB_M_19011506_473.jpg

GTX100297 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
7.5% SDS PAGE
GTX100297 diluted at 1:1000

GTX100297_39428_IFA_19011506_609.jpg

GTX100297 ICC/IF Image

ERp57 antibody [C3], C-term detects PDIA3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: PDIA3 protein stained by ERp57 antibody [C3], C-term (GTX100297) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf