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ERp57 antibody [MaP.Erp57]

Anti-ERp57 antibody [MaP.Erp57] used in Western Blot (WB). GTX13506

Cat. No. GTX13506

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MaP.Erp57

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human, Mouse, Dog, Hamster, Pig, Monkey
Package
50 μg ($399)
See all ERp57 products

APPLICATION

Application Note

IP: Use at a concentration of 10 μg/ml. WB: Use at a concentration of 0.5 μg/ml. This concentration is sufficient for the detection of ERp57 in 20μg of HeLa heat shocked cell lysate by colorimetric analysis. Predicted molecular weight: 61 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

57 kDa. ( Note )

Positive Control

HeLa Heat Shocked Cell Lysate

PROPERTIES

Form

Liquid

Buffer

5mM Sodium Phosphate pH7.2, 25mM Sodium Chloride, 0.05mM PMSF, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

Protein G purified
This antibody is affinity purified.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein disulfide isomerase family A member 3 , ER60 , ERp57 , ERp60 , ERp61 , GRP57 , GRP58 , HEL-S-269 , HEL-S-93n , HsT17083 , P58 , PI-PLC

Cellular Localization

Endoplasmic reticulum

Background

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-ERp57 antibody [MaP.Erp57] used in Western Blot (WB). GTX13506

GTX13506 WB Image

Western blot analysis of ERP57 in human cell lysates using a 1:1000 dilution of GTX13506

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399