APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , HCT116 , Neuro2A , GL261 , C8D30 , 293T , A431 , HeLa , PC-12 , Rat2
Product Note
Knockdown/Knockout & overexpression validation were supported by customer review data.
Predict Reactivity
Rabbit, Sheep, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10720538
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein disulfide isomerase family A member 3 , ER60 , ERp57 , ERp60 , ERp61 , GRP57 , GRP58 , HEL-S-269 , HEL-S-93n , HsT17083 , P58 , PI-PLC
Cellular Localization
Endoplasmic reticulum lumen , Melanosome
Background
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113719 ICC/IF Image
ERp57 antibody detects ERp57 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ERp57 protein stained by ERp57 antibody (GTX113719) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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GTX113719 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with ERp57 antibody (GTX113719) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX113719 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody (GTX113719) diluted at 1:500.
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GTX113719 WB Image
ERp57 antibody detects ERp57 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody (GTX113719) diluted by 1:10000.
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GTX113719 WB Image
Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
7.5% SDS PAGE
GTX113719 diluted at 1:10000
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GTX113719 IHC-P Image
ERp57 antibody detects ERp57 protein at cytoplasm on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
ERp57 antibody (GTX113719) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113719 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ERp57(GTX113719) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113719 WB Image
Sample (30 ug of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX113719 diluted at 1:1000
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REFERENCE
REVIEW
ERp57 antibody
Cat. No. GTX113719
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Rating
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( Average 4 based on 2 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 2 users reviews)
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Date :
Anonymous submitted on 02-Nov-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
293T, HeLa cells
Amount used :
10 μg
Blocking :
Blocking One, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 27-Nov-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
Caco2 cells
Amount used :
20μg
Blocking :
5% milk in PBST, 25°C, 1.5Hr
Primary Antibody :
1:1000, 4°C, 16Hr
