GeneTex
United States (US)

ERp57 antibody

GTX17012_1336_WB_18121410_852.jpg

Cat No. GTX17012

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX17012-PEP).

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EEKPKKKKKAQEDL, from the C Terminus of the protein sequence according to NP_005304.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Disulfide Isomerase Family A Member 3,Er60,Erp57,Erp60,Erp61,Grp57,Grp58,Hel-S-269,Hel-S-93N,Hst17083,P58,Pi-Plc,Pdia3

Cellular Localization

Endoplasmic reticulum

Background

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX17012_1336_WB_18121410_852.jpg

GTX17012 WB Image

WB analysis of HepG2 lysate using GTX17012 ERp57 antibody.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer