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ERp57 antibody

Anti-ERp57 antibody used in Western Blot (WB). GTX32592
Anti-ERp57 antibody used in IHC (Paraffin sections) (IHC-P). GTX32592
Anti-ERp57 antibody used in IHC (Paraffin sections) (IHC-P). GTX32592

Cat. No. GTX32592

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all ERp57 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 206-505 of human PDIA3 (NP_005304.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein disulfide isomerase family A member 3 , ER60 , ERp57 , ERp60 , ERp61 , GRP57 , GRP58 , HEL-S-269 , HEL-S-93n , HsT17083 , P58 , PI-PLC

Cellular Localization

Endoplasmic reticulum

Background

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. This protein also functions as a molecular chaperone that prevents the formation of protein aggregates. [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-ERp57 antibody used in Western Blot (WB). GTX32592

GTX32592 WB Image

WB analysis of various sample lysates using GTX32592 ERp57 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ERp57 antibody used in IHC (Paraffin sections) (IHC-P). GTX32592

GTX32592 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX32592 ERp57 antibody.
Dilution : 1:100

Anti-ERp57 antibody used in IHC (Paraffin sections) (IHC-P). GTX32592

GTX32592 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32592 ERp57 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399