GeneTex
United States (US)

ESE1 antibody [C2C3], C-term

GTX100250_WB_19011506_302.jpg
GTX100250_39456_20151223_IHC-P_19011506_440.jpg
GTX100250_IHC_19011506_426.jpg
GTX100250_39456_IFA_19011506_510.jpg

Cat No. GTX100250

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , Molt-4 , Raji

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ESE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EPR-1 antibody, ERT antibody, ESE-1 antibody, ESX antibody, ELF3 antibody, "ets domain transcription factor, serine box (epithelial-specific) antibody", "E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific) antibody", epithelium-restricted Ets protein ESX antibody, E74-like factor 3 (ets domain transcription factor) antibody, epithelial-restricted with serine box antibody, ETS-related transcription factor Elf-3 antibody, epithelium-specific Ets transcription factor 1 antibody, "E74-like factor 3 (ets domain transcription factor, epithelial-specific ) antibody"

Cellular Localization

Cytoplasm , Nucleus

Background

Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.

Database

DATA IMAGES
GTX100250_WB_19011506_302.jpg

GTX100250 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX100250 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100250_39456_20151223_IHC-P_19011506_440.jpg

GTX100250 IHC-P Image

ESE1 antibody [C2C3], C-term detects ESE1 protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
ESE1 antibody [C2C3], C-term (GTX100250) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100250_IHC_19011506_426.jpg

GTX100250 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ESE1 (GTX100250) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100250_39456_IFA_19011506_510.jpg

GTX100250 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ESE1(GTX100250) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169