APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ESE1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240782
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
E74 like ETS transcription factor 3 , EPR-1 , ERT , ESE-1 , ESX
Cellular Localization
Cytoplasm , Nucleus
Background
Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter. Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity. Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway. May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.
Database
Research Area
DATA IMAGES
![Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250](/templates/default/js/lightbox/images/loading.gif "Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250")
|
GTX100250 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using ESE1 (GTX100250) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX100250 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ESE1 antibody [C2C3], C-term (GTX100250) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX100250 IHC-P Image
ESE1 antibody [C2C3], C-term detects ESE1 protein at cytoplasm in human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
ESE1 antibody [C2C3], C-term (GTX100250) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX100250 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using ESE1(GTX100250) antibody at 1:500 dilution.
|
REFERENCE
There are currently no references for ESE1 antibody [C2C3], C-term (GTX100250). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ESE1 antibody [C2C3], C-term (GTX100250). Be the first to share your experience with this product.
![Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250](/upload/website/prouct_img/small/GTX100250/GTX100250_IHC_w_23053123_424.webp "Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250")
![Anti-ESE1 antibody [C2C3], C-term used in Western Blot (WB). GTX100250](/upload/website/prouct_img/small/GTX100250/GTX100250_39456_20211015_WB_TPM_watermark_w_23053123_509.webp "Anti-ESE1 antibody [C2C3], C-term used in Western Blot (WB). GTX100250")
![Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250](/upload/website/prouct_img/small/GTX100250/GTX100250_39456_20151223_IHC-P_w_23053123_679.webp "Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250")
![Anti-ESE1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100250](/upload/website/prouct_img/small/GTX100250/GTX100250_39456_IFA_w_23053123_737.webp "Anti-ESE1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100250")
![Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250](/upload/website/prouct_img/normal/GTX100250/GTX100250_IHC_w_23053123_424.webp "Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250")
![Anti-ESE1 antibody [C2C3], C-term used in Western Blot (WB). GTX100250](/upload/website/prouct_img/normal/GTX100250/GTX100250_39456_20211015_WB_TPM_watermark_w_23053123_509.webp "Anti-ESE1 antibody [C2C3], C-term used in Western Blot (WB). GTX100250")
![Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250](/upload/website/prouct_img/normal/GTX100250/GTX100250_39456_20151223_IHC-P_w_23053123_679.webp "Anti-ESE1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100250")
![Anti-ESE1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100250](/upload/website/prouct_img/normal/GTX100250/GTX100250_39456_IFA_w_23053123_737.webp "Anti-ESE1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100250")