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ESPN antibody, N-term

KO/KD
Anti-ESPN antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81675
Anti-ESPN antibody, N-term used in Flow cytometry (FACS). GTX81675
Anti-ESPN antibody, N-term used in Western Blot (WB). GTX81675
Anti-ESPN antibody, N-term used in Western Blot (WB). GTX81675
Anti-ESPN antibody, N-term used in Western Blot (WB). GTX81675
Anti-ESPN antibody, N-term used in Western Blot (WB). GTX81675
Anti-ESPN antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81675

Cat. No. GTX81675

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all ESPN products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 17-45 amino acids from the N-terminal region of human ESPN.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

RRID

AB_11173993

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

espin , DFNB36 , LP2654

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Mutations in this gene are associated with autosomal recessive neurosensory deafness, and autosomal dominant sensorineural deafness without vestibular involvement. [provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-ESPN antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81675

GTX81675 IHC-P Image

IHC-P analysis of human testis tissue using GTX81675 ESPN antibody, N-term.

Anti-ESPN antibody, N-term used in Flow cytometry (FACS). GTX81675

GTX81675 FACS Image

FACS analysis of 293 cells using GTX81675 ESPN antibody, N-term.
Green : primary antibody
Blue : negative control

Anti-ESPN antibody, N-term used in Western Blot (WB). GTX81675

GTX81675 WB Image

WB analysis of SK-BR-3 whole cell lysate using GTX81675 ESPN antibody, N-term.
Dilution : 1:1000

Anti-ESPN antibody, N-term used in Western Blot (WB). GTX81675

GTX81675 WB Image

WB analysis of 293 cell lysate (35ug/lane) using GTX81675 ESPN antibody, N-term.

Anti-ESPN antibody, N-term used in Western Blot (WB). GTX81675

GTX81675 WB Image

KO/KD

The data was published in the journal Cancer Cell Int in 2018. PMID: 30618491

Anti-ESPN antibody, N-term used in Western Blot (WB). GTX81675

GTX81675 WB Image

KO/KD

The data was published in the journal Cancer Cell Int in 2018. PMID: 30618491

Anti-ESPN antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX81675

GTX81675 IHC-P Image

The data was published in the journal Cancer Cell Int in 2018. PMID: 30618491

REFERENCE

Application Reference

Li SH et al. Cancer Cell Int 2018; 18 : 219 An actin-binding protein ESPN is an independent prognosticator and regulates cell growth for esophageal squamous cell carcinoma.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399