GeneTex

  • Country / Location Selection

United States (US)

ESRP1 antibody [27H12]

Anti-ESRP1 antibody [27H12] used in Western Blot (WB). GTX48761
Anti-ESRP1 antibody [27H12] used in Western Blot (WB). GTX48761

Cat. No. GTX48761

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 27H12

Isotype

 IgG2b

Application

 WB, ELISA

Reactivity

 Mouse
Package
50 μg ($399)
See all ESRP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ELISA 1:750000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Full length recombinant mouse Esrp-1 fusion protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial splicing regulatory protein 1 , 2210008M09Rik , A630065D16 , BC031468 , Rbm35a

Cellular Localization

Nucleus

Background

Epithelial splicing regulatory protein 1 (Esrp-1) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). EsrpP-1 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Database

Research Area

DATA IMAGES

Anti-ESRP1 antibody [27H12] used in Western Blot (WB). GTX48761

GTX48761 WB Image

Anti-ESRP-1 by western blot shows detection of ESRP-1 in transfected 293T cell extracts (lane 2, arrowhead). Lanes 1 and 3 contain GFP-transfected- and ESRP2-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 μg ysate. Primary antibody was used at a 1:1000 dilution in PBST plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse secondary antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers.

Anti-ESRP1 antibody [27H12] used in Western Blot (WB). GTX48761

GTX48761 WB Image

WB analysis of transfected 293T whole cell lysate using GTX48761 ESRP1 antibody [27H12].
Lane 1 : GFP
Lane 2 : ESRP1
Lane 3 : ESRP2
Loading : 5 μg
Dilution : 1:1000

REFERENCE

There are currently no references for ESRP1 antibody [27H12] (GTX48761). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for ESRP1 antibody [27H12] (GTX48761). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399