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ESRP1 antibody

KO/KD Orthogonal
Anti-ESRP1 antibody used in Western Blot (WB). GTX131373

Cat. No. GTX131373

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ESRP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

HCT116 nuclear extract

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ESRP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886471

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial splicing regulatory protein 1 , DFNB109 , RBM35A , RMB35A

Cellular Localization

Nucleus

Background

ESPR1 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ESRP1 antibody used in Western Blot (WB). GTX131373

GTX131373 WB Image

Orthogonal

HCT116 whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ESRP1 antibody (GTX131373) diluted at 1:1000.

REFERENCE

Application Reference

Singh B et al. Oncotarget 2021; 12 (7) : 626-637 Inhibition of resistant triple-negative breast cancer cells with low-dose 6-mercaptopurine and 5-azacitidine.
Application : WB
Reactivity : Human
Singh B et al. Oncotarget 2019; 10 (38) : 3681-3693 Evaluation of 6-mercaptopurine in a cell culture model of adaptable triple-negative breast cancer with metastatic potential.
Application : WB
G?kmen-Polar Y et al. EMBO Rep 2019; 20 (2) Splicing factor ESRP1 controls ER-positive breast cancer by altering metabolic pathways.
Application : WB
Reactivity : Mouse
Tornillo G et al. Cell Rep 2018; 25 (13) : 3674-3692.e10 Dual Mechanisms of LYN Kinase Dysregulation Drive Aggressive Behavior in Breast Cancer Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169