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ESRP2 antibody

Anti-ESRP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123665
Anti-ESRP2 antibody used in Western Blot (WB). GTX123665

Cat. No. GTX123665

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ESRP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ESRP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163920

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

epithelial splicing regulatory protein 2 , RBM35B

Cellular Localization

Nucleus

Background

ESPR2 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ESRP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123665

GTX123665 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ESRP2(GTX123665) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-ESRP2 antibody used in Western Blot (WB). GTX123665

GTX123665 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HepG2
7.5% SDS PAGE
GTX123665 diluted at 1:1000

REFERENCE

Application Reference

Munkley J et al. Elife 2019; 8 Androgen-regulated transcription of ESRP2 drives alternative splicing patterns in prostate cancer.
Application : WB
Reactivity : Human
Jennifer Munkley et al. bioRxiv 2019; (Epub) Androgen-regulated transcription of ESRP2 drives alternative splicing patterns in prostate cancer.
Application : WB
Reactivity : Human
Groulx JF et al. Cancers (Basel) 2018; 10 (2) MYC Regulates α6 Integrin Subunit Expression and Splicing Under Its Pro-Proliferative ITGA6A Form in Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169