GeneTex
United States (US)

ETEA antibody [N3C3]

GTX115680_40310_IFA_18121410_763.jpg
GTX115680_40310_IHC_18121410_478.jpg
GTX115680_40310_WB_18121410_799.jpg

Cat No. GTX115680

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , *MEF ,

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETEA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fas Associated Factor Family Member 2,Etea,Ubxd8,Ubxn3B,Faf2

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is highly expressed in peripheral blood of patients with atopic dermatitis (AD), compared to normal individuals. It may play a role in regulating the resistance to apoptosis that is observed in T cells and eosinophils of AD patients. [provided by RefSeq]

Database

DATA IMAGES
GTX115680_40310_IFA_18121410_763.jpg

GTX115680 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ETEA(GTX115680) antibody at 1:200 dilution.

GTX115680_40310_IHC_18121410_478.jpg

GTX115680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hs578T xenograft, using ETEA(GTX115680) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115680_40310_WB_18121410_799.jpg

GTX115680 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115680 diluted at 1:1000

REVIEW

ETEA antibody [N3C3] Cat No. GTX115680

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 15-Jul-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : MEF cells
Amount used : 10μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169