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ETEA antibody [N3C3]

KO/KD
Anti-ETEA antibody [N3C3] used in Western Blot (WB). GTX115680
Anti-ETEA antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115680
Anti-ETEA antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115680

Cat. No. GTX115680

3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ETEA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETEA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624542

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fas associated factor family member 2 , ETEA , UBXD8 , UBXN3B

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is highly expressed in peripheral blood of patients with atopic dermatitis (AD), compared to normal individuals. It may play a role in regulating the resistance to apoptosis that is observed in T cells and eosinophils of AD patients. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ETEA antibody [N3C3] used in Western Blot (WB). GTX115680

GTX115680 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115680 diluted at 1:1000

Anti-ETEA antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115680

GTX115680 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using ETEA(GTX115680) antibody at 1:200 dilution.

Anti-ETEA antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX115680

GTX115680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hs578T xenograft, using ETEA(GTX115680) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for ETEA antibody [N3C3] (GTX115680). Be the first to share your publications with this product.
Submit a Reference

REVIEW

ETEA antibody [N3C3] Cat. No. GTX115680

Rating
( Average 4.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 3 users reviews)
Anti-ETEA antibody [N3C3] used in Western Blot (WB). GTX115680
Date : Anonymous submitted on 06-Feb-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293T cells with/without FAF2 KO treatment
Primary Antibody : 1:1000, 4°C, >12Hr
Anti-ETEA antibody [N3C3] used in Western Blot (WB). GTX115680
Date : Anonymous submitted on 06-Feb-2024
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells with/without FAF2 KO treatment
Primary Antibody : 1:1000, 4°C, >12Hr
Anti-ETEA antibody [N3C3] used in Western Blot (WB). GTX115680
Date : Anonymous submitted on 15-Jul-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : MEF cells
Amount used : 10μg
Blocking : 5% skim milk in TBS, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169