GeneTex
United States (US)

ETFA antibody, C-term

GTX88023_2555_WB_18122102_281.jpg

Cat No. GTX88023

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.05-0.2μg/ml
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88023-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_000117.1; NP_001121188.1).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QVADYGIVADLFK, from the C Terminus of the protein sequence according to NP_000117.1; NP_001121188.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Electron Transfer Flavoprotein Subunit Alpha,Ema,Ga2,Madd,Etfa

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88023_2555_WB_18122102_281.jpg

GTX88023 WB Image

WB analysis of human heart lysate using GTX88023 ETFA antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329