GeneTex
United States (US)

ETFA antibody

KO/KD Overexpression
GTX105155_40723_20180914_WB_KO_watermark_19011506_103.jpg
GTX105155_40723_20150910_IP_19011506_656.jpg
GTX105155_40723_20141024_IHC_M_3_19011506_609.jpg
GTX105155_40723_IHC_19011506_123.jpg
GTX105155_40723_WB_19011506_200.jpg
GTX105155_40723_IFA_19011506_306.jpg
GTX105155_40723_20180928_WB_B_19011506_531.jpg
GTX105155_40723_20141024_IHC_M_2_19011506_129.jpg
GTX105155_40723_WB_R_19011506_506.jpg
GTX105155_40723_20141024_IHC_M_19011506_685.jpg

Cat No. GTX105155

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

293T , A431 , HeLa , PC-12

Predict Reactivity

Bovine, Cat, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EMA antibody, GA2 antibody, MADD antibody, ETFA antibody, electron transfer flavoprotein, alpha polypeptide antibody, electron transfer flavoprotein alpha-subunit antibody, electron transfer flavoprotein subunit alpha, mitochondrial antibody, alpha-ETF antibody, glutaric aciduria II antibody, electron-transfer-flavoprotein, alpha polypeptide antibody

Cellular Localization

Mitochondrion matrix

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105155_40723_20180914_WB_KO_watermark_19011506_103.jpg

GTX105155 WB Image

KO/KD

Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX105155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105155_40723_20150910_IP_19011506_656.jpg

GTX105155 IP Image

Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody (GTX105155).
Western blot analysis was performed using ETFA antibody (GTX105155).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX105155_40723_20141024_IHC_M_3_19011506_609.jpg

GTX105155 IHC-P Image

ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105155_40723_IHC_19011506_123.jpg

GTX105155 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ETFA(GTX105155) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105155_40723_WB_19011506_200.jpg

GTX105155 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
10% SDS PAGE
GTX105155 diluted at 1:1000

GTX105155_40723_IFA_19011506_306.jpg

GTX105155 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ETFA(GTX105155) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX105155_40723_20180928_WB_B_19011506_531.jpg

GTX105155 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX105155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105155_40723_20141024_IHC_M_2_19011506_129.jpg

GTX105155 IHC-P Image

ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105155_40723_WB_R_19011506_506.jpg

GTX105155 WB Image

ETFA antibody detects ETFA protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10 % SDS-PAGE
ETFA antibody (GTX105155) dilution: 1:1000

GTX105155_40723_20141024_IHC_M_19011506_685.jpg

GTX105155 IHC-P Image

ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169