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ETFA antibody

KO/KD Overexpression
Anti-ETFA antibody used in Western Blot (WB). GTX105155
Anti-ETFA antibody used in Western Blot (WB). GTX105155
Anti-ETFA antibody used in Western Blot (WB). GTX105155
Anti-ETFA antibody used in IHC (Paraffin sections) (IHC-P). GTX105155
Anti-ETFA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105155
Anti-ETFA antibody used in IHC (Paraffin sections) (IHC-P). GTX105155
Anti-ETFA antibody used in Immunoprecipitation (IP). GTX105155
Anti-ETFA antibody used in IHC (Paraffin sections) (IHC-P). GTX105155
Anti-ETFA antibody used in Western Blot (WB). GTX105155
Anti-ETFA antibody used in IHC (Paraffin sections) (IHC-P). GTX105155

Cat. No. GTX105155

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ETFA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431 , HeLa , PC-12 , ETFA-transfected 293T

Predict Reactivity

Bovine, Cat, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167407

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

electron transfer flavoprotein subunit alpha , EMA , GA2 , MADD

Cellular Localization

Mitochondrion matrix

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ETFA antibody used in Western Blot (WB). GTX105155

GTX105155 WB Image

KO/KD

Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX105155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ETFA antibody used in Western Blot (WB). GTX105155

GTX105155 WB Image

ETFA antibody detects ETFA protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
ETFA antibody (GTX105155) dilution: 1:1000

Anti-ETFA antibody used in Western Blot (WB). GTX105155

GTX105155 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX105155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ETFA antibody used in IHC (Paraffin sections) (IHC-P). GTX105155

GTX105155 IHC-P Image

ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ETFA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105155

GTX105155 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ETFA(GTX105155) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-ETFA antibody used in IHC (Paraffin sections) (IHC-P). GTX105155

GTX105155 IHC-P Image

ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ETFA antibody used in Immunoprecipitation (IP). GTX105155

GTX105155 IP Image

Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody (GTX105155).
Western blot analysis was performed using ETFA antibody (GTX105155).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-ETFA antibody used in IHC (Paraffin sections) (IHC-P). GTX105155

GTX105155 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ETFA(GTX105155) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ETFA antibody used in Western Blot (WB). GTX105155

GTX105155 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
10% SDS PAGE
GTX105155 diluted at 1:1000

Anti-ETFA antibody used in IHC (Paraffin sections) (IHC-P). GTX105155

GTX105155 IHC-P Image

ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Suzuki K et al. J Biol Chem 2023; 299 (10) : 105210 CLPX regulates mitochondrial fatty acid β-oxidation in liver?cells.
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REVIEW

ETFA antibody Cat. No. GTX105155

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-ETFA antibody used in Western Blot (WB). GTX105155
Date : Anonymous submitted on 16-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 5 μg
Blocking : 5% Skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:3000, 25°C, 1Hr
Figure Note : Image courtesy of Suzuki Ko from Iwate Medical University.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169