Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX105155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
10% SDS PAGE
GTX105155 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ETFA(GTX105155) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody (GTX105155).
Western blot analysis was performed using ETFA antibody (GTX105155).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

ETFA antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using ETFA(GTX105155) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

ETFA antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
ETFA antibody (GTX105155) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX105155) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ETFA antibody detects ETFA protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
ETFA antibody (GTX105155) dilution: 1:1000