Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ETFA antibody detects ETFA protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ETFA antibody (GTX114392) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ETFA antibody detects ETFA protein at mitochondria on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ETFA antibody (GTX114392) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ETFA antibody detects ETFA protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
ETFA antibody (GTX114392) dilution: 1:1000

ETFA antibody detects ETFA protein at mitochondria on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
ETFA antibody (GTX114392) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ETFA antibody detects ETFA protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
10 % SDS-PAGE
ETFA antibody (GTX114392) dilution: 1:1000

ETFA antibody detects ETFA protein at mitochondria on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ETFA antibody (GTX114392) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ETFA antibody detects ETFA protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted by 1:1000.

Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody (GTX114392).
Western blot analysis was performed using ETFA antibody (GTX114392).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunofluorescence analysis of methanol-fixed HeLa, using ETFA(GTX114392) antibody at 1:200 dilution.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ETFA antibody (GTX114392) at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:500.