APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
C8D30 , PC-12 , ETFA-transfected 293T
Predict Reactivity
Bovine, Cat, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10619314
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
electron transfer flavoprotein subunit alpha , EMA , GA2 , MADD
Cellular Localization
Mitochondrion matrix
Background
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX114392 WB Image
Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114392 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114392 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using ETFA(GTX114392) antibody at 1:200 dilution.
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GTX114392 IP Image
Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 μg of ETFA antibody (GTX114392).
Western blot analysis was performed using ETFA antibody (GTX114392).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX114392 WB Image
ETFA antibody detects ETFA protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted by 1:1000.
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GTX114392 IHC-P Image
ETFA antibody detects ETFA protein at mitochondria on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ETFA antibody (GTX114392) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX114392 WB Image
ETFA antibody detects ETFA protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
10 % SDS-PAGE
ETFA antibody (GTX114392) dilution: 1:1000
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GTX114392 IHC-P Image
ETFA antibody detects ETFA protein at mitochondria on mouse urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded mouse urinary bladder.
ETFA antibody (GTX114392) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX114392 WB Image
ETFA antibody detects ETFA protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10 % SDS-PAGE
ETFA antibody (GTX114392) dilution: 1:1000
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GTX114392 IHC-P Image
ETFA antibody detects ETFA protein at mitochondria on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ETFA antibody (GTX114392) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX114392 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:500.
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GTX114392 IHC Image
Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ETFA antibody (GTX114392) at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)
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GTX114392 IHC-P Image
ETFA antibody detects ETFA protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
ETFA antibody (GTX114392) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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