GeneTex
United States (US)

ETFB (aa 152-165) antibody, Internal

GTX88025_2553_WB_18122102_329.jpg
GTX88025_1066_IHC-P_18122102_547.jpg

Cat No. GTX88025

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
IHC-P 3-5μg/ml

Note :

IHC-P
Human Liver shows mitochondrial staining in hepatocytes.

Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88025-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). Amino acid numbering in the name above refers to NP_001976.1).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QVTLEGDKLKVERE, from the internal region of the protein sequence according to NP_001976.1; NP_001014763.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Electron Transfer Flavoprotein Subunit Beta,Fp585,Madd,Etfb

Background

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88025_2553_WB_18122102_329.jpg

GTX88025 WB Image

WB analysis of human liver lysate using GTX88025 ETFB (aa 152-165) antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX88025_1066_IHC-P_18122102_547.jpg

GTX88025 IHC-P Image

IHC-P analysis of human liver using GTX88025 ETFB (aa 152-165) antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

Package List Price ($)
$ 329