GeneTex
United States (US)

ETFB (aa 231-243) antibody, C-term

GTX88024_1067_IHC-P_18122102_236.jpg
GTX88024_2554_WB_18122102_210.jpg

Cat No. GTX88024

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
IHC-P 3-5μg/ml
IHC Assay dependent

Note :

Human Liver shows mitochondrial staining in hepatocytes.
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88024-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-PQRTAGVKVETTE, from the C Terminus of the protein sequence according to NP_001976.1; NP_001014763.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MADD Antibody , FP585 Antibody , ETFB Antibody

Background

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
Show More +

Database

Research Area

DATA IMAGES
GTX88024_1067_IHC-P_18122102_236.jpg

GTX88024 IHC-P Image

IHC-P analysis of human liver using GTX88024 ETFB (aa 231-243) antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88024_2554_WB_18122102_210.jpg

GTX88024 WB Image

WB analysis of mouse liver lysate using GTX88024 ETFB (aa 231-243) antibody, C-term.
Dilution : 0.01µg/ml
Loading : 35µg protein in RIPA buffer