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ETFB antibody [N1C3]

Anti-ETFB antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109189
Anti-ETFB antibody [N1C3] used in Western Blot (WB). GTX109189
Anti-ETFB antibody [N1C3] used in Western Blot (WB). GTX109189

Cat. No. GTX109189

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ETFB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brain , Molt-4

Predict Reactivity

Rat, Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETFB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950234

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

electron transfer flavoprotein subunit beta , FP585 , MADD

Cellular Localization

Mitochondrion matrix

Background

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ETFB antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109189

GTX109189 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-ETF(GTX109189) antibody at 1:200 dilution.

Anti-ETFB antibody [N1C3] used in Western Blot (WB). GTX109189

GTX109189 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX109189 diluted at 1:10000

Anti-ETFB antibody [N1C3] used in Western Blot (WB). GTX109189

GTX109189 WB Image

Sample (30 ug of whole cell lysate)
A: MOLT4 (GTX27912)
12% SDS PAGE
GTX109189 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169