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ETFB antibody [N1C3]

Anti-ETFB antibody [N1C3] used in Western Blot (WB). GTX109189
Anti-ETFB antibody [N1C3] used in Western Blot (WB). GTX109189
Anti-ETFB antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109189

Cat No. GTX109189

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
See all ETFB products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETFB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

electron transfer flavoprotein subunit beta , FP585 , MADD

Cellular Localization

Mitochondrion matrix

Background

This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-ETFB antibody [N1C3] used in Western Blot (WB). GTX109189

GTX109189 WB Image

Sample (30 ug of whole cell lysate)
A: MOLT4 (GTX27912)
12% SDS PAGE
GTX109189 diluted at 1:1000

Anti-ETFB antibody [N1C3] used in Western Blot (WB). GTX109189

GTX109189 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX109189 diluted at 1:10000

Anti-ETFB antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109189

GTX109189 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-ETF(GTX109189) antibody at 1:200 dilution.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169