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ETHE1 antibody

Anti-ETHE1 antibody used in Western Blot (WB). GTX109095
Anti-ETHE1 antibody used in Western Blot (WB). GTX109095
Anti-ETHE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109095
Anti-ETHE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109095

Cat. No. GTX109095

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ETHE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

HepG2 , Jurkat , Raji

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ETHE1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166226

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ETHE1 persulfide dioxygenase , HSCO , YF13H12

Cellular Localization

Cytoplasm , Nucleus , Mitochondrion matrix

Background

This gene encodes a protein that is expressed in the thyroid. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ETHE1 antibody used in Western Blot (WB). GTX109095

GTX109095 WB Image

ETHE1 antibody detects ETHE1 protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
15 % SDS-PAGE
ETHE1 antibody (GTX109095) dilution: 1:500

Anti-ETHE1 antibody used in Western Blot (WB). GTX109095

GTX109095 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX109095 diluted at 1:5000

Anti-ETHE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109095

GTX109095 ICC/IF Image

ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: ETHE1 protein stained by ETHE1 antibody (GTX109095) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ETHE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109095

GTX109095 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using ETHE1(GTX109095) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Santos SS et al. Antioxidants (Basel) 2023; 12 (3) Role of Cystathionine β-Synthase and 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation, Migration, and Bioenergetics of Murine Breast Cancer Cells.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169