GeneTex
United States (US)

ETHE1 antibody

GTX115707_40296_IFA_18121410_112.jpg
GTX115707_40296_IHC_18121410_392.jpg
GTX115707_40296_WB_M_liver_18121410_299.jpg
GTX115707_40296_20150410_IFA_18121410_426.jpg

Cat No. GTX115707

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETHE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ethe1, Persulfide Dioxygenase,Hsco,Yf13H12,Ethe1

Cellular Localization

Cytoplasm , Nucleus , Mitochondrion matrix

Background

This gene encodes a protein that is expressed in the thyroid. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115707_40296_IFA_18121410_112.jpg

GTX115707 ICC/IF Image

ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: ETHE1 protein stained by ETHE1 antibody (GTX115707) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115707_40296_IHC_18121410_392.jpg

GTX115707 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ETHE1(GTX115707) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115707_40296_WB_M_liver_18121410_299.jpg

GTX115707 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX115707 diluted at 1:1000

GTX115707_40296_20150410_IFA_18121410_426.jpg

GTX115707 ICC/IF Image

ETHE1 antibody detects ETHE1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ETHE1 protein stained by ETHE1 antibody (GTX115707) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169