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ETNK1 antibody [N2C3]

KO/KD
Anti-ETNK1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105887
Anti-ETNK1 antibody [N2C3] used in Western Blot (WB). GTX105887

Cat. No. GTX105887

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ETNK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.94 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ETNK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950235

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ethanolamine kinase 1 , EKI , EKI 1 , EKI1 , Nbla10396

Cellular Localization

Cytoplasm

Background

This gene encodes an ethanolamine kinase, which functions in the first committed step of the phosphatidylethanolamine synthesis pathway. This cytosolic enzyme is specific for ethanolamine and exhibits negligible kinase activity on choline. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ETNK1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105887

GTX105887 IHC-P Image

ETNK1 antibody [N2C3] detects ETNK1 protein at cytosol on H358 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H358 xenograft.
ETNK1 antibody [N2C3] (GTX105887) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ETNK1 antibody [N2C3] used in Western Blot (WB). GTX105887

GTX105887 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
B:Hep G2 (GTX27900)
10% SDS PAGE
GTX105887 diluted at 1:1000

REFERENCE

Application Reference

Shan S et al. J Agric Food Chem 2020; 68 (31) : 8295-8307 Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.
Application : WB
Reactivity : Human
Osawa T et al. Cell Rep 2019; 29 (1) : 89-103.e7 Phosphoethanolamine Accumulation Protects Cancer Cells under Glutamine Starvation through Downregulation of PCYT2.
Application : WB
Reactivity : Human
Doria ML et al. FASEB J 2014; Fatty acid and phospholipid biosynthetic pathways are regulated throughout mammary epithelial cell differentiation and correlate to breast cancer survival.
Application : WB, IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169